LOCUS       40924_23449       11661 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Gateway binary vector pGWB614 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11661)
  AUTHORS   Nakamura S, Mano S, Tanaka Y, Ohnishi M, Nakamori C, Araki M, Niwa 
            T, Nishimura M, Kaminaka H, Nakagawa T, Sato Y, Ishiguro S.
  TITLE     Gateway binary vectors with the bialaphos resistance gene, bar, as a
            selection marker for plant transformation
  JOURNAL   Biosci. Biotechnol. Biochem. 74 (6), 1315-1319 (2010)
  PUBMED    20530878
REFERENCE   2  (bases 1 to 11661)
  AUTHORS   Tanaka Y, Nakamura S, Ishiguro S, Nakagawa T.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JAN-2010) Contact:Tsuyoshi Nakagawa Shimane 
            University, Center for Integrated Research in Science; 1060 
            Nishikawatsu, Matsue, Shimane 690-8504, Japan
REFERENCE   3  (bases 1 to 11661)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11661)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci. 
            Biotechnol. Biochem."; date: "2010"; volume: "74"; issue: "6"; 
            pages: "1315-1319"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-JAN-2010) Contact:Tsuyoshi Nakagawa Shimane University, Center 
            for Integrated Research in Science; 1060 Nishikawatsu, Matsue, 
            Shimane 690-8504, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     constructed using pPZP221.
FEATURES             Location/Qualifiers
     source          1..11661
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(54..78)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    1179..1303
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1340..1370
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             1424..2080
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             2425..2727
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(2771..2895)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2924..3013
                     /codon_start=1
                     /label=3xHA
                     /note="three tandem HA epitope tags"
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      3047..3299
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3333..3349)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3357..3373
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3381..3411)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3426..3447)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     terminator      complement(3483..3735)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(3898..4446)
                     /codon_start=1
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(4498..4677)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(5183..5207)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5728..6516
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6765..7353
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(7539..7679)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8023..8217)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8286..9356)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9788..10414)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag agttatcaac aagtttgtac aaaaaagctg
     1201 aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac
     1261 agactacata atactgtaaa acacaacata tccagtcact atggcggccc ctcgaggggc
     1321 cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg tgtggatttt
     1381 gagttaggat ccgtcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac
     1441 tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca
     1501 gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa
     1561 gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct
     1621 gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga
     1681 tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg
     1741 gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg
     1801 ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc
     1861 agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt
     1921 cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc
     1981 gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa
     2041 tgaattacaa cagtactgcg atgagtggca gggcggggcg taaagatctg gatccggctt
     2101 actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata
     2161 tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca
     2221 gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg
     2281 gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag
     2341 cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg
     2401 ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa aagagagagc
     2461 cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg
     2521 gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga actttacccg
     2581 gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg
     2641 gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac
     2701 gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct
     2761 gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt
     2821 atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt
     2881 cttgtacaaa gtggttgata acagcgggtt aattaacatc ttttacccat acgatgttcc
     2941 tgactatgcg ggctatccct atgacgtccc ggactatgca ggatcctatc catatgacgt
     3001 tccagattac gctgctcagt gcagcgctta gagctcgaat ttccccgatc gttcaaacat
     3061 ttggcaataa agtttcttaa gattgaatcc tgttgccggt cttgcgatga ttatcatata
     3121 atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga cgttatttat
     3181 gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga tagaaaacaa
     3241 aatatagcgc gcaaactagg ataaattatc gcgcgcggtg tcatctatgt tactagatcg
     3301 ggaattggtt ccggaaccaa ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     3361 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     3421 gcctaatgag tgagctaact cacattaggc tgaattaggc gcgcctattt ctattaattc
     3481 ccgatctagt aacatagatg acaccgcgcg cgataattta tcctagtttg cgcgctatat
     3541 tttgttttct atcgcgtatt aaatgtataa ttgcgggact ctaatcataa aaacccatct
     3601 cataaataac gtcatgcatt acatgttaat tattacatgc ttaacgtaat tcaacagaaa
     3661 ttatatgata atcatcgcaa gaccggcaac aggattcaat cttaagaaac tttattgcca
     3721 aatgtttgaa cgatcgggga aattcgagct ggggacgtcc ccagctcggt accccctcct
     3781 gggcacggtc gtacgcgatc cgccgcggtt ccacctcacc attgcgggta tgccagttga
     3841 gttcgatgag ttcccgtacc agctccaact cgggcgatgc cacgcgcact ccgttcagat
     3901 ctcggtgacg ggcaggaccg gacggggcgg taccggcagg ctgaagtcca gctgccagaa
     3961 acccacgtca tgccagttcc cgtgcttgaa gccggccgcc cgcagcatgc cgcggggggc
     4021 atatccgagc gcctcgtgca tgcgcacgct cgggtcgttg ggcagcccga tgacagcgac
     4081 cacgctcttg aagccctgtg cctccaggga cttcagcagg tgggtgtaga gcgtggagcc
     4141 cagtcccgtc cgctggtggc ggggggagac gtacacggtc gactcggccg tccagtcgta
     4201 ggcgttgcgt gccttccagg ggcccgcgta ggcgatgccg gcgacctcgc cgtccacctc
     4261 ggcgacgagc cagggatagc gctcccgcag acggacgagg tcgtccgtcc actcctgcgg
     4321 ttcctgcggc tcggtacgga agttgaccgt gcttgtctcg atgtagtggt tgacgatggt
     4381 gcagaccgcc ggcatgtccg cctcggtggc acggcggatg tcggccgggc gtcgttctgg
     4441 gctcatatct tattgccccc cgggatcaga tctggattga gagtgaatat gagactctaa
     4501 ttggataccg aggggaattt atggaacgtc agtggagcat ttttgacaag aaatatttgc
     4561 tagctgatag tgaccttagg cgacttttga acgcgcaata atggtttctg acgtatgtgc
     4621 ttagctcatt aaactccaga aacccgcggc tgagtggctc cttcaacgtt gcggttctgt
     4681 cagttccaaa cgtaaaacgg cttgtcccgc gtcatcggcg ggggtcataa cgtgactccc
     4741 ttaattctcc gctcatgatc ttgatcccct gcgccatcag atccttggcg gcaagaaagc
     4801 catccagttt actttgcagg gcttcccaac cttaccagag ggcgccccag ctggcaattc
     4861 cggttcgctt gctgtccata aaaccgccca gtctagctat cgccatgtaa gcccactgca
     4921 agctacctgc tttctctttg cgcttgcgtt ttcccttgtc cagatagccc agtagctgac
     4981 attcatccgg ggtcagcacc gtttctgcgg actggctttc tacgtgttcc gcttccttta
     5041 gcagcccttg cgccctgagt gcttgcggca gcgtgaagct gatccgtcga gattaataga
     5101 aataaattca gcctaattcg gcgttaattc agtacattaa aaacgtccgc aatgtgttat
     5161 taagttgtct aagcgtcaat ttgtttacac cacaatatat cctgccacca gccagccaac
     5221 agctccccga ccggcagctc ggcacaaaat caccactcga tacaggcagc ccatcagtcc
     5281 gggacggcgt cagcgggaga gccgttgtaa ggcggcagac tttgctcatg ttaccgatgc
     5341 tattcggaag aacggcaact aagctgccgg gtttgaaaca cggatgatct cgcggagggt
     5401 agcatgttga ttgtaacgat gacagagcgt tgctgcctgt gatcaattcg ggcacgaacc
     5461 cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc
     5521 aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg
     5581 gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg
     5641 cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc
     5701 agtcgcccta aaacaaagtt aaacatcatg ggggaagcgg tgatcgccga agtatcgact
     5761 caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta
     5821 catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg
     5881 gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg
     5941 gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt
     6001 gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa
     6061 tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg
     6121 gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag
     6181 gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg
     6241 ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc
     6301 cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg
     6361 gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat
     6421 cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccac
     6481 tacgtgaaag gcgagatcac caaggtagtc ggcaaataat gtctagctag aaattcgttc
     6541 aagccgacgc cgcttcgcgg cgcggcttaa ctcaagcgtt agatgcacta agcacataat
     6601 tgctcacagc caaactatca ggtcaagtct gcttttatta tttttaagcg tgcataataa
     6661 gccctacaca aattgggaga tatatcatgc atgaccaaaa tcccttaacg tgagttttcg
     6721 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     6781 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     6841 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     6901 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     6961 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     7021 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     7081 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     7141 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     7201 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     7261 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     7321 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     7381 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     7441 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     7501 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt
     7561 acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat
     7621 gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc
     7681 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     7741 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     7801 caccgaaacg cgcgaggcag ggtgccttga tgtgggcgcc ggcggtcgag tggcgacggc
     7861 gcggcttgtc cgcgccctgg tagattgcct ggccgtaggc cagccatttt tgagcggcca
     7921 gcggccgcga taggccgacg cgaagcggcg gggcgtaggg agcgcagcga ccgaagggta
     7981 ggcgcttttt gcagctcttc ggctgtgcgc tggccagaca gttatgcaca ggccaggcgg
     8041 gttttaagag ttttaataag ttttaaagag ttttaggcgg aaaaatcgcc ttttttctct
     8101 tttatatcag tcacttacat gtgtgaccgg ttcccaatgt acggctttgg gttcccaatg
     8161 tacgggttcc ggttcccaat gtacggcttt gggttcccaa tgtacgtgct atccacagga
     8221 aagagacctt ttcgaccttt ttcccctgct agggcaattt gccctagcat ctgctccgta
     8281 cattaggaac cggcggatgc ttcgccctcg atcaggttgc ggtagcgcat gactaggatc
     8341 gggccagcct gccccgcctc ctccttcaaa tcgtactccg gcaggtcatt tgacccgatc
     8401 agcttgcgca cggtgaaaca gaacttcttg aactctccgg cgctgccact gcgttcgtag
     8461 atcgtcttga acaaccatct ggcttctgcc ttgcctgcgg cgcggcgtgc caggcggtag
     8521 agaaaacggc cgatgccggg atcgatcaaa aagtaatcgg ggtgaaccgt cagcacgtcc
     8581 gggttcttgc cttctgtgat ctcgcggtac atccaatcag ctagctcgat ctcgatgtac
     8641 tccggccgcc cggtttcgct ctttacgatc ttgtagcggc taatcaaggc ttcaccctcg
     8701 gataccgtca ccaggcggcc gttcttggcc ttcttcgtac gctgcatggc aacgtgcgtg
     8761 gtgtttaacc gaatgcaggt ttctaccagg tcgtctttct gctttccgcc atcggctcgc
     8821 cggcagaact tgagtacgtc cgcaacgtgt ggacggaaca cgcggccggg cttgtctccc
     8881 ttcccttccc ggtatcggtt catggattcg gttagatggg aaaccgccat cagtaccagg
     8941 tcgtaatccc acacactggc catgccggcc ggccctgcgg aaacctctac gtgcccgtct
     9001 ggaagctcgt agcggatcac ctcgccagct cgtcggtcac gcttcgacag acggaaaacg
     9061 gccacgtcca tgatgctgcg actatcgcgg gtgcccacgt catagagcat cggaacgaaa
     9121 aaatctggtt gctcgtcgcc cttgggcggc ttcctaatcg acggcgcacc ggctgccggc
     9181 ggttgccggg attctttgcg gattcgatca gcggccgctt gccacgattc accggggcgt
     9241 gcttctgcct cgatgcgttg ccgctgggcg gcctgcgcgg ccttcaactt ctccaccagg
     9301 tcatcaccca gcgccgcgcc gatttgtacc gggccggatg gtttgcgacc gctcacgccg
     9361 attcctcggg cttgggggtt ccagtgccat tgcagggccg gcagacaacc cagccgctta
     9421 cgcctggcca accgcccgtt cctccacaca tggggcattc cacggcgtcg gtgcctggtt
     9481 gttcttgatt ttccatgccg cctcctttag ccgctaaaat tcatctactc atttattcat
     9541 ttgctcattt actctggtag ctgcgcgatg tattcagata gcagctcggt aatggtcttg
     9601 ccttggcgta ccgcgtacat cttcagcttg gtgtgatcct ccgccggcaa ctgaaagttg
     9661 acccgcttca tggctggcgt gtctgccagg ctggccaacg ttgcagcctt gctgctgcgt
     9721 gcgctcggac ggccggcact tagcgtgttt gtgcttttgc tcattttctc tttacctcat
     9781 taactcaaat gagttttgat ttaatttcag cggccagcgc ctggacctcg cgggcagcgt
     9841 cgccctcggg ttctgattca agaacggttg tgccggcggc ggcagtgcct gggtagctca
     9901 cgcgctgcgt gatacgggac tcaagaatgg gcagctcgta cccggccagc gcctcggcaa
     9961 cctcaccgcc gatgcgcgtg cctttgatcg cccgcgacac gacaaaggcc gcttgtagcc
    10021 ttccatccgt gacctcaatg cgctgcttaa ccagctccac caggtcggcg gtggcccata
    10081 tgtcgtaagg gcttggctgc accggaatca gcacgaagtc ggctgccttg atcgcggaca
    10141 cagccaagtc cgccgcctgg ggcgctccgt cgatcactac gaagtcgcgc cggccgatgg
    10201 ccttcacgtc gcggtcaatc gtcgggcggt cgatgccgac aacggttagc ggttgatctt
    10261 cccgcacggc cgcccaatcg cgggcactgc cctggggatc ggaatcgact aacagaacat
    10321 cggccccggc gagttgcagg gcgcgggcta gatgggttgc gatggtcgtc ttgcctgacc
    10381 cgcctttctg gttaagtaca gcgataacct tcatgcgttc cccttgcgta tttgtttatt
    10441 tactcatcgc atcatatacg cagcgaccgc atgacgcaag ctgttttact caaatacaca
    10501 tcaccttttt agacggcggc gctcggtttc ttcagcggcc aagctggccg gccaggccgc
    10561 cagcttggca tcagacaaac cggccaggat ttcatgcagc cgcacggttg agacgtgcgc
    10621 gggcggctcg aacacgtacc cggccgcgat catctccgcc tcgatctctt cggtaatgaa
    10681 aaacggttcg tcctggccgt cctggtgcgg tttcatgctt gttcctcttg gcgttcattc
    10741 tcggcggccg ccagggcgtc ggcctcggtc aatgcgtcct cacggaaggc accgcgccgc
    10801 ctggcctcgg tgggcgtcac ttcctcgctg cgctcaagtg cgcggtacag ggtcgagcga
    10861 tgcacgccaa gcagtgcagc cgcctctttc acggtgcggc cttcctggtc gatcagctcg
    10921 cgggcgtgcg cgatctgtgc cggggtgagg gtagggcggg ggccaaactt cacgcctcgg
    10981 gccttggcgg cctcgcgccc gctccgggtg cggtcgatga ttagggaacg ctcgaactcg
    11041 gcaatgccgg cgaacacggt caacaccatg cggccggccg gcgtggtggt gtcggcccac
    11101 ggctctgcca ggctacgcag gcccgcgccg gcctcctgga tgcgctcggc aatgtccagt
    11161 aggtcgcggg tgctgcgggc caggcggtct agcctggtca ctgtcacaac gtcgccaggg
    11221 cgtaggtggt caagcatcct ggccagctcc gggcggtcgc gcctggtgcc ggtgatcttc
    11281 tcggaaaaca gcttggtgca gccggccgcg tgcagttcgg cccgttggtt ggtcaagtcc
    11341 tggtcgtcgg tgctgacgcg ggcatagccc agcaggccag cggcggcgct cttgttcatg
    11401 gcgtaatgtc tccggttcta gtcgcaagta ttctacttta tgcgactaaa acacgcgaca
    11461 agaaaacgcc aggaaaaggg cagggcggca gcctgtcgcg taacttagga cttgtgcgac
    11521 atgtcgtttt cagaagacgg ctgcactgaa cgtcagaagc cgactgcact atagcagcgg
    11581 aggggttgga tcaaagtact ttgatcccga ggggaaccct gtggttggca tgcacataca
    11641 aatggacgaa cggataaacc t
//
